Strain Data Sheet

RBRC05757

Strain Information

Image
BRC No.RBRC05757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spry4<tm1Ayos>
Former Common nameSprouty4 KO, B6-Spry4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionSprouty4 (Spry4) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal mandible morphology. Although a significant fraction of homozygotes die shortly after birth due to mandible defects, the remainder are viable and fertile, but postnatal growth is reduced for most homozygous mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Sprouty2 and Sprouty4 are essential for embryonic morphogenesis and regulation of FGF signaling.
Taniguchi K, Ayada T, Ichiyama K, Kohno R, Yonemitsu Y, Minami Y, Kikuchi A, Maehara Y, Yoshimura A
Biochem. Biophys. Res. Commun., 352, 896-902 (2007). 17156747

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spry4
MGI:1345144		sprouty RTK signaling antagonist 418Spry4<tm1Ayos>
MGI:3699499		targeted mutation 1, Akihiko Yoshimura
  • hypogonadotropic hypogonadism 17 with or without anosmia(MedGEN)

  • hypogonadotropic hypogonadism 7 with or without anosmia(MedGEN)
    • neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal incisor morphology(MP:0005358)

  • abnormal mandible morphology(MP:0000458)

  • decreased body weight(MP:0001262)

  • malocclusion(MP:0000120)

  • polysyndactyly(MP:0004083)
    • more 3 phenotypes
  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • respiratory system phenotype(MP:0005388)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Spry4 genomic DNA, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophy.s Res. Commun., 352, 896-902 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkihiko Yoshimura (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data