Strain Data Sheet

RBRC05760

Strain Information

Image
BRC No.RBRC05760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spred1<tm1.1Ayos>
Former Common nameSpred1 floxed, B6-Spred1 floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Keio University School of Medicine. A neo cassette flanked by FRT sites was removed by Flp-mediated recombination.
Strain descriptionSpred1 gene floxed mice. Exon 7 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
miR126 positively regulates mast cell proliferation and cytokine production through suppressing Spred1.
Ishizaki T, Tamiya T, Taniguchi K, Morita R, Kato R, Okamoto F, Saeki K, Nomura M, Nojima Y, Yoshimura A
Genes Cells., 16, 803-814 (2011). 21668589

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site2Frt
Spred1
MGI:2150016		sprouty protein with EVH-1 domain 1, related sequence2Spred1<tm1.1Ayos>
MGI:5436575		targeted mutation 1.1, Akihiko Yoshimura
  • Legius syndrome(DisGeNET, MedGEN)
    • loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • craniofacial phenotype(MP:0005382)

  • increased mast cell degranulation(MP:0008764)

  • increased mast cell number(MP:0000324)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Spred1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 16, 803-814 (2011). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkihiko Yoshimura (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data