Strain Data Sheet

RBRC05761

Strain Information

Image
BRC No.RBRC05761
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rftn1<tm1Ayos>
Former Common nameRaftlin KO, B6-Rftn1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionRaftlin (Rftn1) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit impaired T cell-mediated immune responses.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
References
A major lipid raft protein raftlin modulates T cell receptor signaling and enhances th17-mediated autoimmune responses.
Saeki K, Fukuyama S, Ayada T, Nakaya M, Aki D, Takaesu G, Hanada T, Matsumura Y, Kobayashi T, Nakagawa R, Yoshimura A
J. Immunol., 182, 5929-5937 (2009). 19414744

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rftn1
MGI:1923688		raftlin lipid raft linker 117Rftn1<tm1Ayos>
MGI:3850829		targeted mutation 1, Akihiko Yoshimura
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal CD4-positive T cell differentiation(MP:0008076)

  • decreased IgG2a level(MP:0008496)

  • decreased interferon-gamma secretion(MP:0008567)

  • decreased interleukin-17 secretion(MP:0008682)

  • decreased interleukin-4 secretion(MP:0008700)
    • more 2 phenotypes
  • decreased splenocyte number(MP:0009339)

  • decreased susceptibility to experimental autoimmune encephalomyelitis(MP:0004800)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Rftn1 genomic DNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 182, 5929-5937 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkihiko Yoshimura (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data