Strain Data Sheet

RBRC05762

Strain Information

Image
BRC No.RBRC05762
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Trafd1<tm1Ayos>
Former Common nameFLN29, B6-Trafd1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionFLN29 (Trafd1) gene knockout mice. Homozygous mutant mice exhibit increased susceptibility to LPS-endotoxin shock and decreased susceptibility to VSV infection.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
FLN29 deficiency reveals its negative regulatory role in the Toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like helicase signaling pathway.
Sanada T, Takaesu G, Mashima R, Yoshida R, Kobayashi T, Yoshimura A
J. Biol. Chem., 283, :33858-33864 (2008). 18849341

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trafd1
MGI:1923551		TRAF type zinc finger domain containing 15Trafd1<tm1Ayos>
MGI:3831676		targeted mutation 1, Akihiko Yoshimura
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased susceptibility to viral infection(MP:0002410)

  • increased circulating alanine transaminase level(MP:0002941)

  • increased circulating interleukin-6 level(MP:0008596)

  • increased interleukin-12 secretion(MP:0008663)

  • increased interleukin-6 secretion(MP:0008705)
    • more 3 phenotypes
  • increased susceptibility to endotoxin shock(MP:0008735)

  • increased susceptibility to induced morbidity/mortality(MP:0009763)

  • increased tumor necrosis factor secretion(MP:0008560)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Trafd1 genomic DNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 283, :33858-33864 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAkihiko Yoshimura (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data