Strain Information | |
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Image | |
BRC No. | RBRC05765 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko> |
Former Common name | H2AZ1/2 floxed, B6-H2AZ1/2 floxed |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed. |
Strain description | H2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development. |
Colony maintenance | H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Histone variant H2A.Z is required for early mammalian development. Faast R, Thonglairoam V, Schulz T C, Beall J, Wells J R, Taylor H, Matthaei K, Rathjen P D, Tremethick D J, Lyons I Curr. Biol., 11, 1183-1187 (2001). 11516949 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/25Room:4-BRack:ESentinel mouse program 2024/08/26Room:4-BRack:ESentinel mouse program 2024/05/27Room:4-BRack:ESentinel mouse program 2024/02/26Room:4-BRack:ESentinel mouse program 2023/11/27Room:4-BRack:ESentinel mouse program 2023/08/30Room:4-BRack:EAdditional tests 2023/08/28Room:4-BRack:ESentinel mouse program 2023/05/29Room:4-BRack:ESentinel mouse program 2023/03/22Room:4-BRack:EAdditional tests 2023/02/27Room:4-BRack:ESentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 11 | Frt | ||||
H2az2 MGI:1924855 | H2A.Z histone variant 2 | 11 | H2az2<tm1.1Hko> MGI:6163638 | targeted mutation 1.1, Haruhiko Koseki | |||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
Frt | yeast FRT (flippase recombination target) site | 3 | Frt | ||||
H2afz MGI:1888388 | H2A.Z variant histone 1 | 3 | H2afz<tm1.1Hko> MGI:6163639 | targeted mutation 1.1, Haruhiko Koseki | |||
loxP | phage P1 loxP | 3 | loxP | ||||
loxP | phage P1 loxP | 3 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | P1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Reda A, Hategan LA, McLean TAB, Creighton SD, Luo JQ, Chen SES, Hua S, Winston S, Reeves I, Padmanabhan A, Dahi TA, Ramzan F, Brimble MA, Murphy PJ, Walters BJ, Stefanelli G, Zovkic IB. Role of the histone variant H2A.Z.1 in memory, transcription, and alternative splicing is mediated by lysine modification. Neuropsychopharmacology 49(8) 1285-1295(2024) 38366138 |
Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, Senatore A, Monks DA, Liu BH, Leary SC, Cheng HM. Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival. Cell Rep 36(11) 109704(2021) 34525369 |
Rispal J, Baron L, Beaulieu JF, Chevillard-Briet M, Trouche D, Escaffit F. The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis. Nat Commun 10(1) 1827(2019) 31015444 |
Shen T, Ji F, Wang Y, Lei X, Zhang D, Jiao J. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. Nucleic Acids Res 46(5) 2290-2307(2018) 29294103 |