Strain Data Sheet

RBRC05769

Strain Information

Image
BRC No.RBRC05769
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(129S6)-Tg(Aif1-tTA)54Kftnk
Former Common nameIba1-tTA, B6-Aif1-tTA Tg#54
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionIba1-tTA transgenic mice carry a tTA under the control of the modified Iba1 promoter. tTA expression is detected in Iba1 positive macrophage lineage cells (microglia in brain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system.
Tanaka K F, Matsui K, Sasaki T, Sano H, Sugio S, Fan K, Hen R, Nakai J, Yanagawa Y, Hasuwa H, Okabe M, Deisseroth K, Ikenaka K, Yamanaka A
Cell Rep., 2, 397-406 (2012). 22854021

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)UNGH
Tg(Aif1-tTA)54Kftnk
MGI:5439166		UNTg(Aif1-tTA)54Kftnk transgene insertion 54, Kenji Tanaka
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse Iba1 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Iba1 promoter, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, Bovine Growth Hormone polyA additional signal
    Research applicationNeurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 2, 397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
    use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data