Strain Data Sheet

RBRC05770

Strain Information

Image
BRC No.RBRC05770
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Pvalb-tTA)1Kftnk
Former Common namePV-tTA, B6-Pvalb-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6, 129 and CBA mixed background.
Strain descriptionParvalbumin-tTA BAC transgenic mice. tTA expression is detected in PV positive cells (cerebellar Purkinje cells and reticular thalamic nuclei in brain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Application of an optogenetic byway for perturbing neuronal activity via glial photostimulation.
Sasaki T, Beppu K, Tanaka K F, Fukazawa Y, Shigemoto R, Matsui K
Proc. Natl. Acad. Sci. USA, 109(50), 20720-20725 (2012). 23185019

Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.
Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka K F
Cell Rep., 36(4):109427(2021). 34320360

Dysfunction of parvalbumin-expressing cells in the thalamic reticular nucleus induces cortical spike-and-wave discharges and an unconscious state.
Manal S Abdelaal, Mitsuharu Midorikawa, Toru Suzuki, Kenta Kobayashi, Norio Takata, Mariko Miyata, Masaru Mimura, Kenji F Tanaka
Brain Commun., 4(2) fcac010 (2022). 35243344

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
Tg(Pvalb-tTA)1Kftnk UNTg(Pvalb-tTA)1Kftnk transgene insertion 1, Kenji Tanaka
loxP phage P1 loxPUNloxP
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse parvalbumin promoter
SV40 polyA additional signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Pvalb genomic DNA (BAC clone RP24-450L20), E. coli tet repressor (tetR), herpes simplex virus (HSV)VP16 activation domain, SV40 polyA additional signal, P1 Phage loxP site, yeast FRT (flipase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A., 109(50), 20720-20725 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
    use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
    DepositorKenji Tanaka (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data