Strain Data Sheet

RBRC05780

Strain Information

Image
BRC No.RBRC05780
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Rufy3<tm1a(EUCOMM)Hmgu>/B08
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Frt yeast FRT (flippase recombination target) site5Frt
Ires internal ribosomal entry site (EMCV)5
Rufy3
MGI:106484
RUN and FYVE domain containing 35Rufy3<tm1a(EUCOMM)Hmgu>
MGI:4946721
targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
SA Splice acceptor5SA
lacZ beta-galactosidase (E. coli)5
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP
neo neomycin resistance gene (E. coli)5 hBactP (human beta actin promoter)
SV40 polyA signal5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Rufy3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hertz NT, Adams EL, Weber RA, Shen RJ, O'Rourke MK, Simon DJ, Zebroski H, Olsen O, Morgan CW, Mileur TR, Hitchcock AM, Sinnott Armstrong NA, Wainberg M, Bassik MC, Molina H, Wells JA, Tessier-Lavigne M.
    Neuronally Enriched RUFY3 Is Required for Caspase-Mediated Axon Degeneration.
    Neuron 103(3) 412-422.e4(2019) 31221560