Strain Information | |
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Image | |
BRC No. | RBRC05780 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6N-Rufy3<tm1a(EUCOMM)Hmgu>/B08 |
Former Common name | |
H-2 Haplotype | |
ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
Background strain | C57BL/6NTac |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 5 | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | 5 | Frt | ||||
Ires | internal ribosomal entry site (EMCV) | 5 | |||||
Rufy3 MGI:106484 | RUN and FYVE domain containing 3 | 5 | Rufy3<tm1a(EUCOMM)Hmgu> MGI:4946721 | targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH | |||
SA | Splice acceptor | 5 | SA | ||||
lacZ | beta-galactosidase (E. coli) | 5 | |||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 5 | hBactP (human beta actin promoter) | ||||
SV40 polyA signal | 5 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Rufy3 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively. |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Hertz NT, Adams EL, Weber RA, Shen RJ, O'Rourke MK, Simon DJ, Zebroski H, Olsen O, Morgan CW, Mileur TR, Hitchcock AM, Sinnott Armstrong NA, Wainberg M, Bassik MC, Molina H, Wells JA, Tessier-Lavigne M. Neuronally Enriched RUFY3 Is Required for Caspase-Mediated Axon Degeneration. Neuron 103(3) 412-422.e4(2019) 31221560 |