Strain Data Sheet

RBRC05795

Strain Information

Image
BRC No.RBRC05795
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(MSCV-EGFP)1Hirai
Former Common nameB6-Tg(MSCV-GFP), B6-MSCV-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Hirokazu Hirai, Gunma University Graduate School of Medicine in 2008. Lentiviral vectors were used to introduce a MSCV-EGFP cassette into the genomes of C57BL/6J mouse embryos.
Strain descriptionMSCV-GFP transgenic mice EXPRESSING a EGFP under the control of the Murine stem cell virus (MSCV) promoter. In the brain, Tg expression is detected in the Purkinje cells and in the brainstem, especially in the pontine nuclei. Tg expression is also detected in several tissues, especially in the thymus, leucocytes, muscle and skin.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References
The murine stem cell virus promoter drives correlated transgene expression in the leukocytes and cerebellar Purkinje cells of transgenic mice.
Oue M, Handa H, Matsuzaki Y, Suzue K, Murakami H, Hirai H
PLoS One, 7, e51015 (2012). 23226450

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP Murine stem cell virus (MSCV) promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMurine stem cell virus (MSCV) promoter, Aequorea victoria EGFP cDNA, HIV Long terminal repeat (LTR)
    Research applicationFluorescent Proteins/lacZ System
    Hematological Research
    Immunology and Inflammation Research
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE must inform the purpose of the use and receive DEPOSITOR's approval in advance. The RECIPIENT can use the BIOLOGICAL RESOURCE as collaborative researches.
    DepositorHirokazu Hirai (Gunma University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data