Strain Information | |
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Image | |
BRC No. | RBRC05796 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | STOCK Tc(HSA21q-MAC1) |
Former Common name | DS-MAC, HSA21-MAC Tc |
H-2 Haplotype | |
ES Cell line | TT2F [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yasuhiro Kazuki, Graduate School of Medical Sciences in 2011. Mixed background. Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile. |
Strain description | Transchromosomic mice. 21q of the human chromosome was transferred into mouse embryonic stem cell by using mouse artificial chromosome (MAC) vectors Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile. |
Colony maintenance | Hemizygote (female) x Wild-type (male) [or Crossing to Slc : B6D2F1]Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile. |
References | A novel and stable mouse artificial chromosome vector. Takiguchi M, Kazuki Y, Hiramatsu K, Abe S, Iida Y, Takehara S, Nishida T, Ohbayashi T, Wakayama T, Oshimura M ACS Synth. Biol., (2012). 23654256A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21. Kazuki Y, Gao F J, Li Y, Moyer A J, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N, Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea L D, Savonenko A, Xiao M, Wu D, Batista D A, Yang J, Qiu Z, Singh N, Richtsmeier J T, Takeuchi T, Oshimura M, Reeves R H Elife, 9:e56223 (2020). 32597754 |
Health Report | |
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Examination Date / Room / Rack | 2024/12/09Room:3-6Rack:CSentinel mouse program 2024/09/09Room:3-6Rack:CSentinel mouse program 2024/06/10Room:3-6Rack:CSentinel mouse program 2024/03/11Room:3-6Rack:CSentinel mouse program 2023/12/11Room:3-6Rack:CSentinel mouse program 2023/09/11Room:3-6Rack:CSentinel mouse program 2023/06/13Room:3-6Rack:CSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
HSA21q | UN | HSA21q | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
MAC1 | UN | MAC1 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 9 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | The Long Arm of Human Chromosome 21, HPRT gene, Bovine growth hormone (BGH) polyA signal, chicken beta-globin insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 large T antigen, SV40 early promoter, SV40 Origin (Ori), polyA signal, P1 Phage loxP site, E. coli ampicilin resistance gene, mouse PGK promoter, E. coli neo resistance gene, E. coli Kan resistance gene, E. coli Cm resistance gene, E. coli hyg resistance gene, jellyfish GFP cDNA, mouse chromosome 11 centromere region |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Mouse Models for Human Disease |
Specific Term and Conditions | 1. The use of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research purpose. 2. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 3. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 4. The RECIPIENT must prior inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA. 5. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Kazuki Y. et al. elife. 2020 Jun 29;9:e56223. doi: 10.7554/eLife.56223. PMID: 32597754 6. RIKEN BRC should report the DEPOSITOR the RECIPIENT’s name, address and organization once a year. |
Depositor | Yasuhiro Kazuki (Tottori University) |
Strain Status | ![]() ![]() |
Strain Availability | Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2021 |
BRC mice in Publications |
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Kazuki Y, Gao FJ, Li Y, Moyer AJ, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N, Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea LD, Savonenko A, Xiao M, Wu D, Batista DA, Yang J, Qiu Z, Singh N, Richtsmeier JT, Takeuchi T, Oshimura M, Reeves RH. A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21. Elife 9 (2020) 32597754 |