Strain Data Sheet

RBRC05796

Strain Information

Image
BRC No.RBRC05796
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tc(HSA21q-MAC1)
Former Common nameDS-MAC, HSA21-MAC Tc
H-2 Haplotype
ES Cell lineTT2F [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasuhiro Kazuki, Graduate School of Medical Sciences in 2011. Mixed background. Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
Strain descriptionTranschromosomic mice. 21q of the human chromosome was transferred into mouse embryonic stem cell by using mouse artificial chromosome (MAC) vectors Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
Colony maintenanceHemizygote (female) x Wild-type (male) [or Crossing to Slc : B6D2F1]Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
References
A novel and stable mouse artificial chromosome vector.
Takiguchi M, Kazuki Y, Hiramatsu K, Abe S, Iida Y, Takehara S, Nishida T, Ohbayashi T, Wakayama T, Oshimura M
ACS Synth. Biol., (2012). 23654256

A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21.
Kazuki Y, Gao F J, Li Y, Moyer A J, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N, Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea L D, Savonenko A, Xiao M, Wu D, Batista D A, Yang J, Qiu Z, Singh N, Richtsmeier J T, Takeuchi T, Oshimura M, Reeves R H
Elife, 9:e56223 (2020). 32597754

Health Report

Examination Date / Room / Rack2025/03/10Room:3-6Rack:CSentinel mouse program
2024/12/09Room:3-6Rack:CSentinel mouse program
2024/09/09Room:3-6Rack:CSentinel mouse program
2024/06/10Room:3-6Rack:CSentinel mouse program
2024/03/21Room:3-6Rack:CAdditional tests
2024/03/11Room:3-6Rack:CSentinel mouse program
2023/12/11Room:3-6Rack:CSentinel mouse program
2023/09/11Room:3-6Rack:CSentinel mouse program
2023/06/13Room:3-6Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HSA21q UNHSA21q CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
MAC1 UNMAC1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brain morphology(MP:0002152)

  • abnormal learning/memory/conditioning(MP:0002063)

  • abnormal platelet cell number(MP:0011898)

  • abnormal postnatal growth/weight/body size(MP:0002089)

  • abnormal spleen morphology(MP:0000689)
    • more 9 phenotypes
  • amyloid beta deposits(MP:0003329)

  • atrioventricular septal defect(MP:0010412)

  • decreased anxiety-related response(MP:0001364)

  • double outlet right ventricle(MP:0000284)

  • increased forebrain volume(MP:0012560)

  • increased spleen white pulp amount(MP:0008478)

  • male infertility(MP:0001925)

  • reproductive system phenotype(MP:0005389)

  • ventricular septal defect(MP:0010402)
    • Detailed phenotype data

    Ordering Information

    Donor DNAThe Long Arm of Human Chromosome 21, HPRT gene, Bovine growth hormone (BGH) polyA signal, chicken beta-globin insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 large T antigen, SV40 early promoter, SV40 Origin (Ori), polyA signal, P1 Phage loxP site, E. coli ampicilin resistance gene, mouse PGK promoter, E. coli neo resistance gene, E. coli Kan resistance gene, E. coli Cm resistance gene, E. coli hyg resistance gene, jellyfish GFP cDNA, mouse chromosome 11 centromere region
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and Conditions1. The use of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research purpose.
    2. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    3. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    4. The RECIPIENT must prior inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA.
    5. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Kazuki Y. et al. elife. 2020 Jun 29;9:e56223. doi: 10.7554/eLife.56223. PMID: 32597754
    6. RIKEN BRC should report the DEPOSITOR the RECIPIENT’s name, address and organization once a year.
    DepositorYasuhiro Kazuki (Tottori University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    Strain AvailabilityCryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2021

    BRC mice in Publications

    Kazuki Y, Gao FJ, Li Y, Moyer AJ, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N, Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea LD, Savonenko A, Xiao M, Wu D, Batista DA, Yang J, Qiu Z, Singh N, Richtsmeier JT, Takeuchi T, Oshimura M, Reeves RH.
    A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21.
    Elife 9 (2020) 32597754