Strain Data Sheet

RBRC05800

Strain Information

Image
BRC No.RBRC05800
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rab33b<tm1Mitf>/3108
Former Common nameRab33B KO, B6-Rab33B KO
H-2 Haplotype
ES Cell lineBA1 [(C57BL/6 x 129/SvEv)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Mitsunori Fukuda, Graduate School of Life Sciences, Tohoku University in 2006. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionRab33B gene knockout mice. Exon 1 was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]Homozygous mutant mice show no obvious abnormality.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
Rab33b
MGI:1330805		RAB33B, member RAS oncogene family3Rab33b<tm1Mitf> targeted mutation 1, Mitsunori Fukuda
  • Smith-McCort dysplasia 2(MedGEN)
    • loxP phage P1 loxP3loxP
    loxP phage P1 loxP3loxP
    neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Phage P1 loxP, Yeast frt sites, mouse phosphoglycerate kinase promoter (PGK promoter), mouse Rab33b genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the BIOLOGICAL RESOURCE.
    DepositorMitsunori Fukuda (Tohoku University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data