Strain Data Sheet
RBRC05821
Strain Information | |
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| Image | |
| BRC No. | RBRC05821 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Camk2a<tm1.1Oyam> |
| Former Common name | B6-CaMKII alpha (K42R), B6-Camk2a KI/K42R |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yoko Yamagata and Yuchio Yanagawa, National Institute for Physiological Sciences in 2003. Derived from TT2 ES cell line (B6CBAF1 derived). The PGK-neo cassette was removed by transient expression of cre recombinase in ES cells. C57BL/6 background (RBRC05821), ICR mixed background (RBRC05636). |
| Strain description | Camk2a/K42R knock-in mice. Ca<2+>/calmodulin-dependent protein kinase IIalpha (CaMKII alpha/ Camk2a) is an enzyme that adds phosphates to a variety of protein substrates to modify their functions. CaMKIIalpha is especially enriched in the hippocampus, and is believed to be an essential mediator of activity-dependent synaptic plasticity and memory functions. We generated a novel kinase-dead knock-in mouse of the CaMKII alpha gene that completely and exclusively lacks its kinase activity by introducing a point mutation (K42R) that prevents ATP binding necessary for phosphorylation reaction. Both heterozygous and homozygous mice are hyperactive, but otherwise indistinguishable from wild-type mice. They are fertile and without any obvious neurological symptoms, except for sporadic observation of mild limbic seizure. Homozygous mice are severely impaired with hippocampal synaptic plasticity and hippocampus-dependent memory formation. Suitable for studies of the mechanisms of LTP and learning and memory, and as a memory-deficit animal model. |
| Colony maintenance | Back crossed to C57BL/6NCrlCrlj for more than 20 generations. |
| References | Kinase-dead knock-in mouse reveals an essential role of kinase activity of Ca2+/calmodulin-dependent protein kinase IIalpha in dendritic spine enlargement, long-term potentiation, and learning. Yamagata Y, Kobayashi S, Umeda T, Inoue A, Sakagami H, Fukaya M, Watanabe M, Hatanaka N, Totsuka M, Yagi T, Obata K, Imoto K, Yanagawa Y, Manabe T, Okabe S J. Neurosci., 29, 7607-7618 (2009). 19515929 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Camk2a MGI:88256 | calcium/calmodulin-dependent protein kinase II alpha | 18 | Camk2a<tm1.1Oyam> MGI:3849851 | targeted mutation 1.1, Yoko Yamagata | |||
| loxP | phage P1 loxP | 18 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 6 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1 Phage loxP site, mouse Camk2a genomic DNA |
| Research application | Cre/loxP system Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 29, 7607-7618 (2009). |
| Depositor | Yoko Yamagata (National Institute for Physiological Sciences) |
| Strain Status |  Frozen sperm |
| Strain Availability | Live mouse (more than 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |