Strain Data Sheet

RBRC05841

Strain Information

Image
BRC No.RBRC05841
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(hOX-tTA)1Ahky
Former Common nameOrexin-tTA, B6-hOX-tTA Tg, C57BL/6-Tg(hOX-tTA)1Ahky
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2008. C57BL/6J background.
Strain descriptionOrexin neuron specific tTA transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
References
Ectopic expression of melanopsin in orexin/hypocretin neurons enables control of wakefulness of mice in vivo by blue light.
Tsunematsu T, Tanaka K F, Yamanaka A, Koizumi A
Neurosci. Res., 75(1):23-8 (2013). 22868039

Influence of inhibitory serotonergic inputs to orexin/hypocretin neurons on the diurnal rhythm of sleep and wakefulness.
Tabuchi S, Tsunematsu T, Kilduff T S, Sugio S, Xu M, Tanaka K F, Takahashi S, Tominaga M, Yamanaka A
Sleep, 36(9):1391-404 (2013). 23997373

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA human orexin (hypocretin) promoter
SV40 polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman orexin (hypocretin) promoter, E. coli Tet repressor (tetR), HSV VP16 activation domain, SV40 polyA
    Research applicationNeurobiology Research
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data