Strain Data Sheet

RBRC05842

Strain Information

Image
BRC No.RBRC05842
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(tetO-ArchT/EGFP)1Ahky
Former Common nameTetO ArchT, B6-tetO-ArchT/GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2011. C57BL/6J background.
Strain descriptionThese transgenic mice express an Archaerhodopsin-T/EGFP fusion protein under the control of the TRE (tetracycline responsive element) promoter.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
References
A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex.
Han X, Chow B Y, Zhou H, Klapoetke N C, Chuong A, Rajimehr R, Yang A, Baratta M V, Winkle J, Desimone R, Boyden E S
Front. Syst. Neurosci., 5:18 (2011). 21811444

Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice.
Tsunematsu T, Tabuchi S, Tanaka K F, Boyden E S, Tominaga M, Yamanaka A
Behav. Brain Res., 255, 64-74 (2013). 23707248

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ArchT Archaerhodopsin-T (Halorubrum strain TP009)UNArchT E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
Frt yeast FRT (flippase recombination target) siteUNFrt
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron, Halorubrum strain TP009 Archaerhodopsin, jellyfish GFP cDNA, SV40 polyA, mouse Beta actin, P1 phage loxP sites, yeast FRT (flipase recomnibation target) site
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data