Strain Information | |
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Image | |
BRC No. | RBRC05843 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky> |
Former Common name | TetO ET/TC, B6-tetO-ChR2/ETTC KI |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Dr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2012. |
Strain description | The tetO-ChR2(E123T/T159C) cassette was inserted into the Actb gene. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl]Homozygous mice are viable and fertile. |
References | High-efficiency channelrhodopsins for fast neuronal stimulation at low light levels. Berndt A, Schoenenberger P, Mattis J, Tye K M, Deisseroth K, Hegemann P, Oertner T G Proc. Natl. Acad. Sci. USA, 108, 7595-600 (2011). 21504945Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation. Tsunematsu T, Ueno T, Tabuchi S, Inutsuka A, Tanaka K F, Hasuwa H, Kilduff T S, Terao A, Yamanaka A J. Neurosci. 34, 6896-6909 (2014). 24828644 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Actb MGI:87904 | actin, beta | 5 | Actb <tm1(tetO-ChR2*E123T/T159C)Ahky> | targeted mutation 1, Akihiro Yamanaka | more 1 Diseases | ||
ChR2 | channelrhodopsin-2 (Chlamydomonas reinhardtii) | 5 | ChR2 | E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron | |||
EYFP | Enhanced Yellow Fluorescent Protein (Aequorea victoria) | 5 | EYFP | ||||
Frt | yeast FRT (flippase recombination target) site | 5 | Frt | ||||
HBB2 | rabbit beta-globin intron | 5 | HBB2 | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
loxP | phage P1 loxP | 5 | loxP | ||||
SV40 polyA | 5 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron, C. reinhardtii Channelrhodopsin2, jellyfish YFP cDNA, SV40 polyA, mouse Beta actin, P1 phage loxP sites, yeast FRT (flipase recomnibation target) site, mouse Actb genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Akihiro Yamanaka (Nagoya University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Dec 2013 |
BRC mice in Publications |
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Suzuki E, Momiyama T. M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons. Eur J Neurosci 53(3) 796-813(2021) 33270289 |