Strain Data Sheet

RBRC05843

Strain Information

Image
BRC No.RBRC05843
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky>
Former Common nameTetO ET/TC, B6-tetO-ChR2/ETTC KI
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2012.
Strain descriptionThe tetO-ChR2(E123T/T159C) cassette was inserted into the Actb gene.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]Homozygous mice are viable and fertile.
References
High-efficiency channelrhodopsins for fast neuronal stimulation at low light levels.
Berndt A, Schoenenberger P, Mattis J, Tye K M, Deisseroth K, Hegemann P, Oertner T G
Proc. Natl. Acad. Sci. USA, 108, 7595-600 (2011). 21504945

Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.
Tsunematsu T, Ueno T, Tabuchi S, Inutsuka A, Tanaka K F, Hasuwa H, Kilduff T S, Terao A, Yamanaka A
J. Neurosci. 34, 6896-6909 (2014). 24828644

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb
MGI:87904
actin, beta5Actb <tm1(tetO-ChR2*E123T/T159C)Ahky> targeted mutation 1, Akihiro Yamanaka
  • autosomal dominant nonsyndromic hearing loss 20(MedGEN)

  • Baraitser-Winter syndrome 1(MedGEN)

  • Baraitser-winter syndrome 2(MedGEN)
  • more 1 Diseases
  • developmental malformations-deafness-dystonia syndrome(MedGEN)
  • ChR2 channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2 E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron
    EYFP Enhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP
    Frt yeast FRT (flippase recombination target) site5Frt
    HBB2 rabbit beta-globin intron5HBB2
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    SV40 polyA5

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron, C. reinhardtii Channelrhodopsin2, jellyfish YFP cDNA, SV40 polyA, mouse Beta actin, P1 phage loxP sites, yeast FRT (flipase recomnibation target) site, mouse Actb genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2013

    BRC mice in Publications

    Suzuki E, Momiyama T.
    M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons.
    Eur J Neurosci 53(3) 796-813(2021) 33270289