Strain Data Sheet

RBRC05844

Strain Information

Image
BRC No.RBRC05844
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Pmch-tTA)BAhky
Former Common nameMCH-tTA, B6-MCH-tTA Tg, C57BL/6-Tg(MCH-tTA)1Ahky
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2010. C57BL/6J background. MCH locus of the BAC RP23-348H6 clone was replaced with tTA.
Strain descriptionMelanin-concentrating hormone (MCH) neuron specific tTA BAC transgenic mice.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg mice are viable and fertile.
References
Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.
Tsunematsu T, Ueno T, Tabuchi S, Inutsuka A, Tanaka K F, Hasuwa H, Kilduff T S, Terao A, Yamanaka A
J. Neurosci., 34(20):6896-909 (2014). 24828644

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse melanin-concentrating hormone (MCH) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse melanin-concentrating hormone (MCH) genomic DNA (mouse BAC PR23-348H6), tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), SV40 polyA, Phage P1 loxP site from BAC vector
    Research applicationNeurobiology Research
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hung CJ, Ono D, Kilduff TS, Yamanaka A.
    Dual orexin and MCH neuron-ablated mice display severe sleep attacks and cataplexy.
    Elife 9 (2020) 32314734