Strain Data Sheet

RBRC05845

Strain Information

Image
BRC No.RBRC05845
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(hOX-Arch/EGFP)1Ahky
Former Common nameOrexin-Arch, B6-hOX-Arch/GFP Tg, B6D2-Tg(hOX-Arch/EGFP)1Ahky
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2009. C57BL/6 and DBA/2 mixed background.
Strain descriptionThese transgenic mice express an Archaerhodopsin/EGFP fusion protein under the control of the Orexin promoter.
Colony maintenanceCarrier x Carrier [or Crossing to Jcl:B6D2F1]Tg homozygous and hemizygous mice are viable and fertile.
References
New approaches for the study of orexin function.
Yamanaka A, Tsunematsu T
J. Neuroendocrinol., 22(7): 818-824 (2010). 20456607

Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice.
Tsunematsu T, Tabuchi S, Tanaka K F, Boyden E S, Tominaga M, Yamanaka A
Behav. Brain Res., 255:64-74 (2013). 23707248

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Arch ArchaerhodopsinUNArch human orexin (hypocretin) promoter
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman orexin (hypocretin) promoter, Halorubrum Archaerhodopsin, jellyfish GFP cDNA, mouse Protamine I polyA
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Williams RH, Tsunematsu T, Thomas AM, Bogyo K, Yamanaka A, Kilduff TS.
    Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy.
    J Neurosci 39(47) 9435-9452(2019) 31628177