Strain Data Sheet

RBRC05846

Strain Information

Image
BRC No.RBRC05846
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Tph2-tTA)1Ahky
Former Common nameTph2-tTA, B6-Tph2-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2010. Tph2 locus of the BAC RP23-112F24 clone was replaced with tTA.
Strain descriptionSerotonin neuron neuron specific tTA BAC transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
References
Optogenetic activation of serotonergic neurons enhances anxiety-like behaviour in mice.
Ohmura Y, Tanaka K F, Tsunematsu T, Yamanaka A, Yoshioka M
Int J Neuropsychopharmacol. 17(11):1777-83 (2014). 24834486

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
loxP phage P1 loxPUNloxP
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse tryptophan hydroxylase 2 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Tet repressor (tetR), HSV VP16 activation domain, SV40 polyA, mouse BAC RP23-112F24 (Phage P1 loxP site, yeast FRT (flipase recombination target) site)
    Research applicationCre/loxP system
    FLP/frt system
    Neurobiology Research
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Dantsuji M, Mochizuki A, Nakayama K, Kanamaru M, Izumizaki M, Tanaka KF, Inoue T, Nakamura S.
    Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice.
    Sci Rep 14(1) 27703(2024) 39533095
    N OKUMA, M KANAMARU, R MORIYA, KF TANAKA, S ARATA, J WATANABE, A YOSHIKAWA, M IZUMIZAKI.
    Optogenetic Stimulation of 5-HT Neurons in the Median Raphe Nucleus Affects Anxiety and Respiration.
    Showa Univ. J. Med. Sci. 31(3) 263-274(2019)