Strain Data Sheet

RBRC05847

Strain Information

Image
BRC No.RBRC05847
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn
Former Common nameEdnrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain descriptionEdnrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter.
Colony maintenanceEdnrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote)
References
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Hosoda K, Hammer R E, Richardson J A, Baynash A G, Cheung J C, Giaid A, Yanagisawa M
Cell, 79, 1267-1276 (1994). 8001159

Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo.
Mackenzie M A, Jordan S A, Budd P S, Jackson I J
Dev. Biol., 192, 99-107 (1997). 9405100

Transgenic expression of the endothelin-B receptor prevents congenital intestinal aganglionosis in a rat model of Hirschsprung disease.
Gariepy C E, Williams S C, Richardson J A, Hammer R E, Yanagisawa M
J. Clin. Invest., 102, 1092-1101 (1998). 9739043

Genomic localization of a Dct-LacZ transgene locus: a simple assay for transgene status.
Takemoto Y, Keighren M, Jackson I J, Yamamoto H
Pigment Cell Res., 19, 644-645 (2006). 17083491

Health Report

Examination Date / Room / Rack2023/04/10Room:3-ARack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ednrb
MGI:102720		endothelin receptor type B14Ednrb<tm1Ywa>
MGI:1857161		targeted mutation 1, Masashi Yanagisawa
  • ABCD syndrome(MedGEN)

  • Hirschsprung disease, susceptibility to, 2(MedGEN)

  • Waardenburg syndrome type 4A(MedGEN)
    • neo neomycin resistance gene (E. coli)14 mouse phosphoglycerate kinase promoter (PGK promoter)
    Ednrb endothelin receptor type B (rat)UNEdnrb human dopamine beta hydroxylase (DBH) promoter
    lacZ beta-galactosidase (E. coli)UN mouse dopachrome tautomerase promoter
    SV40 splice and polyadenylation signalsUN

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal hair follicle melanocyte morphology(MP:0004381)

  • white spotting(MP:0002938)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMus msuculus PGK promoter, Escherichia coli Neomycin resistance gene, Rattus norvegicus Endothelin receptor beta, Homo sapiens DBH-promoter, Escherichia coli LacZ, Mus msuculus Dct promoter, SV40 splice and polyadenylation signals
    Research applicationFluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT.
    DepositorMasashi Kato (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data