Strain Data Sheet
RBRC05849
Strain Information | |
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| Image | |
| BRC No. | RBRC05849 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Dkk3<tm1.1> |
| Former Common name | C57BL/6J-Dkk3 KO(wt/KO/Cre), Dkk3 KO(wt/KO/Cre) |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masaki Watabe and Hiromi Kumon, Okayama University Graduate School of Medicine and Kazuhiko Ochiai, Faculty of Veterinary Science, School of Veterinary Nursing and Technology. Bruce4 ES cells were used. Exon 5 to 6 and PGK-neo cassette were removed by crossing with a Cre mice (B6 background). |
| Strain description | REIC/Dkk3 gene knockout mice. Exons 5 and 6 were removed. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]Homozygous mutant mice are viable and fertile. |
| References | A REIC gene shows down-regulation in human immortalized cells and human tumor-derived cell lines. Tsuji T, Miyazaki M, Sakaguchi M, Inoue Y, Namba M Biochem. Biophys. Res. Commun., 268, 20-24 (2000). 10652205Down-regulation of inhibition of differentiation-1 via activation of activating transcription factor 3 and Smad regulates REIC/Dickkopf-3-induced apoptosis. Kashiwakura Y, Ochiai K, Watanabe M, Abarzua F, Sakaguchi M, Takaoka M, Tanimoto R, Nasu Y, Huh N H, Kumon H Cancer Res., 68, 8333-8341 (2008). 18922905Molecular simulation analysis of the structure complex of C2 domains of DKK family members and beta-propeller domains of LRP5/6: explaining why DKK3 does not bind to LRP5/6. Fujii Y, Hoshino T, Kumon H Acta Med. Okayama, 68(2):63-78 (2014). 24743782 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Dkk3 MGI:1354952 | dickkopf WNT signaling pathway inhibitor 3 | 7 | Dkk3<tm1.1> | targeted mutation 1.1, Hiromi Kumon | |||
| loxP | phage P1 loxP | 7 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 loxP site, mouse Dkk3 genomic DNA (NCBI.No.BC046304) |
| Research application | Cancer Research Cre/loxP system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Acta Med. Okayama, 68(2):63-78 (2014). |
| Depositor | Hiromi Kumon (Momotaro-Gene Inc.) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |