Strain Data Sheet

RBRC05853

Strain Information

Image
BRC No.RBRC05853
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Igh<tm1(AK23)>
Former Common nameAK23 IgH KI, B6-IgH KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain descriptionThe endogenous DQ53 and JH1-4 elements were replaced by a anti-Dsg3 VDJ (AK23) H chain.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NTac]
References
IgM to IgG Class Switching Is a Necessary Step for Pemphigus Phenotype Induction in Desmoglein 3-Specific B Cell Receptor Knock-in Mouse.
Hisashi Nomura, Naoko Wada, Hayato Takahashi, Yuko Kase, Jun Yamagami, Shohei Egami, Hisato Iriki, Miho Mukai, Aki Kamata, Hiromi Ito, Hideki Fujii, Tomoyuki Ishikura, Haruhiko Koseki, Takashi Watanabe, Taketo Yamada, Osamu Ohara, Shigeo Koyasu, Masayuki Amagai
J. Immunol. 208: 582-593 (2022). 34996836

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Igh
MGI:96442		immunoglobulin heavy chain complex12Igh <tm1(AK23)> targeted mutation 1
neo neomycin resistance gene (E. coli)12 mouse Phosphoglycerate kinase (PGK) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse immunoglobulin enhancer and promoter, mouse immunoglobulin heavy chain genes, mouse Phosphoglycerate kinase (PGK) promoter, E. coli transposon Tn5 neomycin resistance gene
    Research applicationImmunology and Inflammation Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol. 208: 582-593. (doi: 10.4049/jimmunol.2100781)
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data