Strain Data Sheet

RBRC05881

Strain Information

Image
BRC No.RBRC05881
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Phf2<tm1.2Yima>
Former Common namePHF2 flox, B6-Phf2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yosuke Okuno and Dr. Yuuki Imai, Institute of Molecular and Cellular Biosciences, The University of Tokyo in 2010. C57BL/6 and 129 mixed. Frt flanked region was removed by crossing with Actb-FLPe mice (B6 background).
Strain descriptionPhf2 gene floxed mice. Exon 6 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice show no obvious abnormality.
References
Epigenetic regulation of adipogenesis by PHF2 histone demethylase.
Okuno Y, Ohtake F, Igarashi K, Kanno J, Matsumoto T, Takada I, Kato S, Imai Y
Diabetes, 62(5):1426-1434 (2013). 23274892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site13Frt
Phf2
MGI:1338034		PHD finger protein 213Phf2<tm1> targeted mutation 1, Shigeaki Kato
loxP phage P1 loxP13loxP
loxP phage P1 loxP13loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal adipose tissue development(MP:0011167)

  • decreased body weight(MP:0001262)

  • decreased bone mass(MP:0004016)

  • decreased muscle weight(MP:0004232)

  • decreased white adipose tissue mass(MP:0014146)
    • more 3 phenotypes
  • increased brain weight(MP:0002176)

  • neonatal lethality(MP:0002058)

  • postnatal growth retardation(MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Phf2 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Diabetes, 62(5):1426-1434 (2013).
    DepositorYuki Imai (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data