Strain Information | |
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Image | |
BRC No. | RBRC05998 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6-Tg(Nes-cre/ERT2)4Imayo |
Former Common name | Nestin-CreERT2 Line4 ; B6-Nes-CreER#4 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. |
Strain description | Nestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is intermediate. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Temporal regulation of Cre recombinase activity in neural stem cells. Imayoshi I, Ohtsuka T, Metzger D, Chambon P, Kageyama R Genesis, 44(5):233-8 (2006). 16652364 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/18Room:4-ARack:HSentinel mouse program 2024/08/19Room:4-ARack:HSentinel mouse program 2024/05/20Room:4-ARack:HSentinel mouse program 2024/02/19Room:4-ARack:HSentinel mouse program 2023/11/20Room:4-ARack:HSentinel mouse program 2023/08/21Room:4-ARack:HSentinel mouse program 2023/05/22Room:4-ARack:HSentinel mouse program 2023/02/20Room:4-ARack:HSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | UN | ERT2 | ||||
cre | Phage P1 Cre recombinase | UN | cre | ||||
SV40 polyA signal | UN |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Rat Nestin promoter, Nestin 2nd intron enhancer, SV40 PolyA signal, Cre recombinase (P1 pharge), human ER ligand binding domain (ERT2) |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 44(5):233-8 (2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K. Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles. Sci Rep 12(1) 17820(2022) 36280775 |