Strain Information | |
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Image | |
BRC No. | RBRC06002 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | ICR.Cg-Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo> |
Former Common name | Rosa26R-Hes1-IRES-EGFP ; ROSA26/Hes1GFP |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR. |
Strain description | Hes1-IRES-EGFP Rosa26 knock-in mice. Expression of Hes1 and EGFP can be induced after Cre-mediated recombination. |
Colony maintenance | Homozygote x Homozygote [or Crossing to Slc:ICR] |
References | Science, 342(6163):1203-8. doi: 10.1126/science.1242366. 24179156 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo>targeted mutation 1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hes1hairy and enhancer of split 1 (Drosophila)6Hes1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 late polyA signal6 |
Ordering Information | |
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Donor DNA | adenovirus splice acceptor sequence (SA), Bacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), SV40 late polyA signal, mouse Hes1 cDNA, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA,bovine GH polyA, mouse Gt(ROSA)26Sor genomic region |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science DOI: 10.1126/science.1242366In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |