Strain Data Sheet
RBRC06002
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC06002 |
| Type | Gene Trap |
| Species | Mus musculus |
| Strain name | ICR.Cg-Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo> |
| Former Common name | Rosa26R-Hes1-IRES-EGFP ; ROSA26/Hes1GFP |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR. |
| Strain description | Hes1-IRES-EGFP Rosa26 knock-in mice. Expression of Hes1 and EGFP can be induced after Cre-mediated recombination. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to Slc:ICR] |
| References | Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R Science, 342(6163):1203-8 (2013). 24179156 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | ||||
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo> | targeted mutation 1, Itaru Imayoshi | |||
| Hes1 | hairy and enhancer of split 1 (Drosophila) | 6 | Hes1 | ||||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| SA | Splice acceptor (adenovirus) | 6 | SA | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
| SV40 late polyA signal | 6 | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | adenovirus splice acceptor sequence (SA), Bacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), SV40 late polyA signal, mouse Hes1 cDNA, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA,bovine GH polyA, mouse Gt(ROSA)26Sor genomic region |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 342(6163):1203-8 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
| Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |