Strain Data Sheet
RBRC06008
Strain Information | |
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| Image | |
| BRC No. | RBRC06008 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | ICR.Cg-Hes5<tm1(venus)Imayo> |
| Former Common name | Venus-Hes5 fusion Knock-in ; Hes5/venus KI |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. TT2 ES cells were used. The mutant mice were crossed to ICR. |
| Strain description | Venus/Hes5 knock-in mice. Venus-Hes5 fusion protein is expressed from Hes5 locus. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] |
| References | Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R Science, 342(6163):1203-8 (2013). 24179156 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| FLAG | FLAG tag (synthetic) | 4 | FLAG | ||||
| Frt | yeast FRT (flippase recombination target) site | 4 | Frt | ||||
| Hes5 MGI:104876 | hes family bHLH transcription factor 5 | 4 | Hes5<tm1(venus)Imayo> | targeted mutation 1, Itaru Imayoshi | |||
| Venus | Yellow Fluorescent Protein (Aequorea victoria) | 4 | Venus | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | FLAG tag (synthetic), Jellyfish venus cDNA, Yeast Frt (flippase recombination target) site, mouse Hes5 genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 342(6163):1203-8 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |