Strain Data Sheet

RBRC06019

Strain Information

Image
BRC No.RBRC06019
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR-Tg(Ascl1-luc2/Ascl1)1Imayo
Former Common nameLuc2-Ascl1 fusion BAC ; Ascl1-luc2/Ascl1 Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain descriptionAscl1 BAC transgenic mice, in which luc2-Ascl1 fusion protein is expressed.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
References
Oscillatory control of factors determining multipotency and fate in mouse neural progenitors.
Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R
Science, 342(6163):1203-8 (2013). 24179156

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
HA human influenza virus HA (hemagglutinin) tag sequenceUNHA
luc2 Photinus pyralis LuciferaseUNluc2

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHA (hemagglutinin) tag sequence, Optimized luciferase (synthetic), Yeast Frt (flippase recombination target) site, mouse Asc11 genomic DNA (BAC clone RP24-130P7)
    Research applicationFLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 342(6163):1203-8 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data