Strain Data Sheet

RBRC06032

Strain Information

Image
BRC No.RBRC06032
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(KPNA1)Yyo>/75
Former Common nameImpa5 KI, Line No:75, 85
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tetsuji Moriyama and Yoshihiro Yoneda, Graduate School of Frontier Biosciences, Osaka University in 2012.
Strain descriptionKpna1 (IPOA5, Impoirtin alpha 5) gene ROSA26 knock-in mice.
Colony maintenance
References
Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging.
Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T
Genesis, 49, 579-590 (2011). 21445964

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(KPNA1)Yyo> targeted mutation 1, Yoshihiro Yoneda
HA human influenza virus HA (hemagglutinin) tag sequence6HA
Kpna1 karyopherin (importin) alpha 16Kpna1
SA Splice acceptor (adenovirus)6SA
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
tpA SV40 ployadenylation sequence(tpA)6tpA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAAdenovirus splice acceptor sequence (SA), Bacteriophage P1 locus of X-over P1 (loxP), E. coli Neomycin resistance gene, Mouse phosphoglycerate kinase promoter (PGK promoter), SV40 ployadenylation sequence (tpA), Human influenza Human influenza hemagglutinin (HA), Mouse Karyopherin alpha1 (KPNA1), Bovine growth hormone polyadenylation sequence, Mouse Gt(ROSA)26Sor genomic region
    Research applicationCell Biology Research
    Cre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 49, 579-590 (2011).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and notify him the research content. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR/DEVELOPER as co-authors.
    DepositorMasahiro Oka (National Institutes of Biomedical Innovation, Health and Nutrition)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data