Strain Data Sheet

RBRC06036

Strain Information

Image
BRC No.RBRC06036
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.1(H2B-mCherry)Osb>
Former Common nameROSA26-H2B-mCherry
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used. Frt flanked neo was removed by transient expression of FLP recombinase in fertilized eggs of conditional MethylRO mice.
Strain descriptionROSA26 H2B-mCherry knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality.
References
Long-term, six-dimensional live-cell imaging for the mouse preimplantation embryo that does not affect full-term development.
Yamagata K, Suetsugu R, Wakayama T
J. Reprod. Dev., 55, 343-350 (2009). 19305125

Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection.
Yamagata K, Suetsugu R, Wakayama T
Hum. Reprod., 24, 2490-2499 (2009). 19574276

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(H2B-mCherry)Osb> targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
SA Splice acceptor (adenovirus)6SA
mCherry Red Fluorescent Protein (Discosoma sp.)6mCherry human H2B promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor (SA), yeast FRT (flipase recombination target) site (F3 site; synthetic variant 3), human H2B genomic DNA, Discosoma sp. mCherry cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationDevelopmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuo Yamagata (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fujiwara K, Inoue T, Kimoto A, Zixian J, Tokuhiro K, Yasukochi Y, Akama TO, Cai CL, Shiojima I, Kimura H, Yoshimura SH, Nakamura T, Hirai M.
    Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress.
    Cell Rep 43(12) 115048(2024) 39656588
    Takahashi H, Nakashima T, Masuda T, Namba M, Sakamoto S, Yamaguchi K, Horimasu Y, Miyamoto S, Iwamoto H, Fujitaka K, Hamada H, Hattori N.
    Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity.
    Stem Cell Res Ther 12(1) 471(2021) 34425896