Strain Information | |
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Image | |
BRC No. | RBRC06037 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6-Pou5f1<tm1(Pou5f1/EGFP)Osb> |
Former Common name | Oct3/4-EGFP knock-in mouse |
H-2 Haplotype | |
ES Cell line | EGR-101 [C57BL/6N] |
Background strain | |
Appearance | |
Strain development | Developed by Jun Ueda, Hitoshi Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-101 ES cells derived from C57BL/6N were used. |
Strain description | Oct3/4-EGFP knock-in mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl]Homozygous mutant mice are probably lethal. |
References | Identification and characterization of subpopulations in undifferentiated ES cell culture. Toyooka Y, Shimosato D, Murakami K, Takahashi K, Niwa H Development, 135, 909-918 (2008). 18263842 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 17 | EGFP | mouse Oct3/4 promoter | |||
IRES | internal ribosomal entry site (EMCV) | 17 | |||||
Pou5f1 MGI:101893 | POU domain, class 5, transcription factor 1 | 17 | Pou5f1<tm1(Pou5f1/EGFP)Osb> | targeted mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
puro | puromycin acyltransferase (Streptomyces alboniger) | 17 | puro |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Oct3/4 genomic DNA, Streptomyces alboniger puromycin-resistance gene, Jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires) |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced. |
Depositor | Kazuo Yamagata (Osaka University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Okamoto K, Fujita H, Okada Y, Shinkai S, Onami S, Abe K, Fujimoto K, Sasaki K, Shioi G, Watanabe TM. Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance. EMBO J e112305(2023) 37609947 |