Strain Data Sheet

RBRC06038

Strain Information

Image
BRC No.RBRC06038
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1(tTA)>/1Kyo
Former Common nameB6-Tet-HCN4#1, B6-Hcn4/tTA KI#1
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040).
Strain descriptionHcn4 tTA knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc] Homozygous mutant mice may be embryonic lethal.
References
Hyperpolarisation-activated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in mice.
Nakashima N, Ishii T M, Bessho Y, Kageyama R, Ohmori H
J. Physiol., 591, 1749-1769 (2013). 23318872

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH human growth hormone gene9GH
Hcn4
MGI:1298209		hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>
MGI:5560747		targeted mutation 1, Noriyuki NakashimaE. coli tet operator, cytomegalovirus CMV promoter
  • Brugada syndrome 8(MedGEN)

  • sick sinus syndrome 2, autosomal dominant(MedGEN)
    • URA3 Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3
    loxP phage P1 loxP9loxP
    loxP phage P1 loxP9loxP
    neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTA
    SV40 polyA signal9

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal nervous system electrophysiology(MP:0002272)

  • abnormal olfactory bulb glomerular layer morphology(MP:0009948)

  • embryonic lethality, complete penetrance(MP:0011092)

  • small olfactory bulb(MP:0002741)
    • Detailed phenotype data

    Ordering Information

    Donor DNAtetracycline transactivator tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), SV40 polyA signal, Phage P1 loxP sites, Herpes simplex virus thymidine kinase promoter, E. coli neomycin resistance gene, Herpes simplex virus thymidine kinase polyA signal, yeast URA3 gene, human growth hormone polyA, tetO5-CMV (E. coli tet operator, cytomegalovirus CMV promoter), mouse Hcn4 genomic DNA
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 591, 1749-1769 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S).
    DepositorHarunori Ohmori (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data