Strain Data Sheet

RBRC06050

Strain Information

Image
BRC No.RBRC06050
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-Cd109)203Michi
Former Common nameCD109-Tg-203
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Sayaka Sobue and Masatoshi Ichihara, College of Life and Health Sciences, Chubu University. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6 over 10 times. Line 203 (BRC06050), line 206 (BRC06051), line 213 (BR06052).
Strain descriptionCAG-CD109 transgenic mice. These mice express the mouse CD109 gene under the control of the CAG promoter. The expression level of the CD109 gene in each line of Tg mice was following order: line 206>213>203.
Colony maintenance
References
Detection of a soluble form of CD109 in serum of CD109 transgenic and tumor xenografted mice.
Sakakura H, Murakumo Y, Mii S, Hagiwara S, Kato T, Asai M, Hoshino A, Yamamoto N, Sobue S, Ichihara M, Ueda M, Takahashi M
PLoS One. 2014 9(1): e83385. 24400073

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd109 CD109 antigenUNCd109 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
FLAG FLAG tag (synthetic)UNFLAG
His His tag (synthetic)UNHis

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Cd109, CMV CMV-IE promoter, Chicken beta-actin promoter, Rabbit beta globin polyA, FLAG tag, His tag
    Research applicationCancer Research
    Hematological Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2014 9(1): e83385.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE, the RECIPIENT must inform the research outline to the DEPOSITOR, discuss the collaborative project and publish a paper co-authored with the DEPOSITOR in the case of collaboration. If the DEPOSITOR and the RECIPIENT do not conduct collaborative study, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form prior to requesting the BIOLOGICAL RESOURCE.
    DepositorMasatomo Ichihara (Chubu University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data