Strain Data Sheet

RBRC06054

Strain Information

Image
BRC No.RBRC06054
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Polk<tm3(mpolk)Csk> Tg(gptdelta)1Nmi
Former Common nameinactivated Polk KI and gpt delta double transgenic mice
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Takehiko Nohmi, National Institute of Health Sciences. Inactivate Polk KI mice were generated by using C57BL/6N derived ES cells. The mutant mice were crossed with the gpt delta Tg mice (C57BL/6J background) (RBRC00158). C57BL/6J and C57BL/6N mixed background.
Strain descriptionInactivate Polk knock-in mice, in which Asp at position 197 and Glu at position 198 of exon 6 were replace with Ala, respectively. This mutant mice also have a gpt delta transgene that serves as a tool for assessing the mutagenicity of environmental chemicals.
Colony maintenanceKI: Heterozygote x Wild-type; Tg: Carrier x Carrier
References
In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.
Takeiri A, Wada N A, Motoyama S, Matsuzaki K, Tateishi H, Matsumoto K, Niimi N, Sassa A, Gruz P, Masumura K, Yamada M, Mishima M, Jishage K I, Nohmi T
DNA Repair, 24, 113-121(2014). 25303778

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Polk
MGI:1349767		polymerase (DNA directed), kappa13Polk<tm3(mpolk)Csk> targeted mutation 3, Chugai Pharmaceutical Co, Ltd
Polk
MGI:1349767		polymerase (DNA directed), kappa13Polk<tm3(mpolk)Csk> targeted mutation 3, Chugai Pharmaceutical Co, Ltd
cat E. coli chloramphenicol acetyltransferase (CAT)17
gpt Delta guanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>
MGI:3612991		transgene insertion 1, Takehito Nohmi
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
red/gam lambda phage red/gam17red/gam

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse inactivated polymerase k cDNA, mouse inactivated polymerase k genomic DNA, lambda phage red/gam Ori, P1 phage loxP sites, lambda 2001, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
    Research applicationCancer Research
    Cre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of the mice with the DEPOSITOR and its co-developer, Chugai Pharmaceutical Co., Ltd. (Contact person: Dr. Akira Takeiri, e-mail, takeiriakr@chugai-pharm.co.jp) and must obtain a prior written consent from them and, if necessary, shall enter into an agreement with them.
    DepositorTakehiko Noumi (National Institute of Health Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data