Strain Data Sheet

RBRC06134

Strain Information

Image
BRC No.RBRC06134
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Oog1*3.9-AcGFP)B
Former Common nameOog1pro3.9 B, B6-Oog1-AcGFP/3.9 Tg#B
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naojiro Minami, Graduate School of Agriculture, Kyoto University and Seiji Kito, Satoshi Tsukamoto, National Institute of Radiological Sciences in 2011. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionOog1-GFP transgenic mice. Oog1 gene is an oocyte-specific gene. AcGFP1-mem reporter is expressed under the control of the 2.7 kb or 3.9 kb upstream regions of the Oog1 gene. 2.7 kb type (RBRC06132), 3.9 kb type (RBRC06133 and RBRC06134).
Colony maintenanceCarrier x Noncarrier [C57BL/6]
References
Oogenesin is a novel mouse protein expressed in oocytes and early cleavage-stage embryos.
Minami N, Aizawa A, Ihara R, Miyamoto M, Ohashi A, Imai H
Biol Reprod 69, 1736-1742 (2003). 12890732

The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice.
Ishida M, Okazaki E, Tsukamoto S, Kimura K, Aizawa A, Kito S, Imai H, Minami N
PLoS One, 8(7):e68686 (2013). 23894331

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
AcGFP1 Green Fluorescent Protein (Aequorea coerulescens)UNAcGFP1 mouse Oog1 promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Oog1 promoter, Aequorea coerulescens AcGFP1-mem cDNA, SV40 PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(7):e68686 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNaojiro Minami (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Komatsu K, Masubuchi S.
    Mouse oocytes connect with granulosa cells by fusing with cell membranes and form a large complex during follicle development.
    Biol Reprod 99(3) 527-535(2018) 29590310