Strain Data Sheet

RBRC06138

Strain Information

Image
BRC No.RBRC06138
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Serpina12<tm1>
Former Common nameVaspin KO, B6-Serpina12 KO, Vaspin-/-
H-2 Haplotype
ES Cell lineBK4 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Jun Wada, Department of Medicine and Clinical Science, Okayama University and Yoichiro Iwakura, Institute of Medical Sciences, University of Tokyo in 2008. 129P2 derived ES cells were used. The mutant mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionVaspin (Serpina12) gene knockout mice. Exon 2 was deleted. Homozygous mutant mice fed with a high-fat-high sucrose diet exhibit increase in body weight, fad pad, liver weight and deterioration of insulin sensitivity.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Vaspin is an adipokine ameliorating ER stress in obesity as a ligand for cell-surface GRP78/MTJ-1 complex.
Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Horiguchi C S, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
Diabetes, 61, 2823-2832, (2012). 22837305

Visceral adipose tissue-derived serine proteinase inhibitor inhibits apoptosis of endothelial cells as a ligand for the cell-surface GRP78/voltage-dependent anion channel complex.
Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
Circ Res., 112, 771-780 (2013). 23307819

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Serpina12
MGI:1915304		serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1212Serpina12<tm1Jwada> targeted mutation 1, Jun Wada
loxP phage P1 loxP12loxP
loxP phage P1 loxP12loxP
neo neomycin resistance gene (E. coli)12 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal fat cell morphology(MP:0009115)

  • homeostasis/metabolism phenotype(MP:0005376)

  • increased body weight(MP:0001260)

  • increased circulating LDL cholesterol level(MP:0000182)

  • increased circulating cholesterol level(MP:0005178)
    • more 6 phenotypes
  • increased circulating leptin level(MP:0005669)

  • increased epididymal fat pad weight(MP:0009288)

  • increased fat cell size(MP:0006094)

  • increased liver triglyceride level(MP:0009355)

  • increased liver weight(MP:0002981)

  • insulin resistance(MP:0005331)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, phage P1 loxP, Mouse Serpina12 genomic DNA
    Research applicationCre/loxP system
    Diabetes and Obesity Research
    Metabolism Research
    Mouse Models for Human Disease
    covid19_progression
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorJun Wada (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data