Strain Data Sheet

RBRC06139

Strain Information

Image
BRC No.RBRC06139
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Fabp4-Serpina12)1
Former Common nameVaspin Tg#1, B6-Serpina12 Tg#1
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Jun Wada, Department of Medicine and Clinical Science, Okayama University and Yoichiro Iwakura, Institute of Medical Sciences, University of Tokyo in 2005. C57BL/6J background.
Strain descriptionVaspin (Serpina12) gene transgenic mice. These mice express the mouse Vaspin gene under the control of the aP2 promoter. Tg mice are protected from diet-induced obesity, glucose intolerance, and hepatic steatosis.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Vaspin is an adipokine ameliorating ER stress in obesity as a ligand for cell-surface GRP78/MTJ-1 complex.
Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Horiguchi C S, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
Diabetes, 61, 2823-2832, (2012). 22837305

Visceral adipose tissue-derived serine proteinase inhibitor inhibits apoptosis of endothelial cells as a ligand for the cell-surface GRP78/voltage-dependent anion channel complex.
Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
Circ Res., 112, 771-780 (2013). 23307819

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (human)UNGH
HBB2 rabbit beta-globin intronUNHBB2
Serpina12 serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12UNSerpina12 mouse fatty acid binding protein 4, adipocyte promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased body weight(MP:0001262)

  • decreased circulating LDL cholesterol level(MP:0000183)

  • decreased circulating insulin level(MP:0002727)

  • decreased circulating leptin level(MP:0005668)

  • decreased epididymal fat pad weight(MP:0009289)
    • more 5 phenotypes
  • decreased liver triglyceride level(MP:0009356)

  • decreased liver weight(MP:0003402)

  • decreased susceptibility to hepatic steatosis(MP:0002310)

  • homeostasis/metabolism phenotype(MP:0005376)

  • increased insulin sensitivity(MP:0002891)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse fatty acid binding protein 4 (Fabp4) adipocyte promoter, mouse SERPINA12 (vaspin) gene cDNA, rabbit beta-globin intron, human GH polyA signal
    Research applicationDiabetes and Obesity Research
    Metabolism Research
    Mouse Models for Human Disease
    covid19_progression
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorJun Wada (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data