Strain Data Sheet

RBRC06142

Strain Information

Image
BRC No.RBRC06142
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Tnfsf11<tm1>
Former Common nameRANKL flox, B6-Tnfsf11 floxed
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Toshio Fumoto and Kyoji Ikeda, National Center for Geriatrics and Gerontology (2009-2013). 129Sv/EvTac derived ES cells were used. Frt flanked neo cassette was removed by crossing with CAG-FLPe (B6). The mutant mice were crossed C57BL/6 OVER 9 times.
Strain descriptionRANKL (Tnfsf11) gene floxed mice. Exons 3 and 4 were flanked by loxP site.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygous mice show no obvious abnormality.
References
Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis.
Fumoto T, Takeshita S, Ito M, Ikeda K
J. Bone. Miner. Res., 29, 830-842 (2014). 24014480

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site14Frt
Tnfsf11
MGI:1100089		tumor necrosis factor (ligand) superfamily, member 1114Tnfsf11<tm1Suna>
MGI:5544614		targeted mutation 1, Sunao Takeshita
  • autosomal recessive osteopetrosis 2(MedGEN)
    • loxP phage P1 loxP14loxP
    loxP phage P1 loxP14loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal bone ossification(MP:0008271)

  • abnormal osteoclast cell number(MP:0004983)

  • abnormal skeleton development(MP:0002113)

  • decreased compact bone mass(MP:0010962)

  • decreased compact bone thickness(MP:0000135)
    • more 3 phenotypes
  • increased bone volume(MP:0010875)

  • osteopetrosis(MP:0000067)

  • osteosclerosis(MP:0005422)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Yeast FRT site, mouse RANKL genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Bone. Miner. Res., 29, 830-842 (2014).
    DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data