Strain Data Sheet

RBRC06145

Strain Information

Image
BRC No.RBRC06145
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB.Cg-Tg(HSA-Slk*K63R)654
Former Common nameHSA K63R line 654, FVB-HAS-SLK/K63R Tg#654
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Luc Sabourin, Ottawa Hospital Research Institute. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F1 fertilized eggs. The mutant mice were crossed to FVB/N for several times.
Strain descriptionTransgene expressing the HA-tagged, dominant negative cDNA (K63R) of SLK, under the control of the HAS promoter. Line 654 (RBRC06145) and line 3405 (RBRC06146). Tg mice exhibit some muscle defects. Line 654 and 3405 Tg mice have a high and low expression level of K63R, respectively. Line 654 Tg mice tend to yield smaller litters and fewer litters than their wild-type or line 3405 counterparts.
Colony maintenanceNoncarrier (female) x Carrier (male) [or Crossing to FVB/NJcl]
References
Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.
Storbeck C J, Al-Zahrani K N, Sriram R, Kawesa S, O'Reilly P, Daniel K, McKay M, Kothary R, Tsilfidis C, Sabourin L A
Skelet Muscle., 3(1):16 (2013). 23815977

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HA human influenza virus HA (hemagglutinin) tag sequenceUNHA
Slk mouse SLK mutated gene (upstream of full-length kinase-inactive SLK bearing a point mutation at lysine 63)UNSlk human skeletal actin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal chromosome morphology(MP:0003702)

  • abnormal muscle fiber morphology(MP:0004087)

  • abnormal myoblast differentiation(MP:0011808)

  • decreased litter size(MP:0001935)

  • decreased skeletal muscle fiber diameter(MP:0009402)
    • more 3 phenotypes
  • delayed muscle development(MP:0000736)

  • embryonic lethality(MP:0008762)

  • reduced vestibular evoked myogenic potential(MP:0004744)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman skeletal actin promoter, mouse SLK mutated gene (upstream of full-length kinase-inactive SLK bearing a point mutation at lysine 63), HA (hemagglutinin) tag sequence
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Skelet Muscle., 3(1):16 (2013).
    DepositorLuc Sabourin (Ottawa Hospital Research Institute)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data