Strain Data Sheet

RBRC06157

Strain Information

Image
BRC No.RBRC06157
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a4b<tm1Csk>
Former Common nameSGLT3b knockout mouse, SGLT3b KO, B6-Slc5a4B KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a4b gene by homologous recombination. C57BL/6N background.
Strain descriptionSlc5a4b (SGLT3b) gene knockout mice. Exon 1 to 2 and part of exon 3 were replaced with a PGK neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slc5a4b
MGI:1890478		solute carrier family 5 (neutral amino acid transporters, system A), member 4b10Slc5a4b<tm1Csk> targeted mutation 1, Chugai Pharmaceutical Co., Ltd.
neo neomycin resistance gene (E. coli)10 mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter (synthetic), E. coli Neomycine resistance gene, (synthetic) poly A signal, mouse Slc5a4b genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
    DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data