Strain Data Sheet

RBRC06158

Strain Information

Image
BRC No.RBRC06158
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Del(10Slc5a4a-Slc5a4b)Csk
Former Common nameSGLT3a/3b double knockout mouse, SGLT3a/3b dKO, B6-10Slc5a4a/4b KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used. C57BL/6N background.
Strain descriptionSlc5a4a (SGLT3a) and Slc5a4b (SGLT3b) genes double knockout mice. Exon 1 to 5 of the Slc5a4b and exon 1 to 2 and part of exon 3 of Slc5a4a were deleted. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-Type [or Crossing to C57BL/6NCrl]
References

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:JSentinel mouse program
2024/01/09Room:3-BRack:JSentinel mouse program
2023/10/10Room:3-BRack:JSentinel mouse program
2023/07/10Room:3-BRack:JSentinel mouse program
2023/04/10Room:3-BRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slc5a4a
MGI:1927848		solute carrier family 5, member 4a10Slc5a4a deletion, Chr 10, Chugai Pharmaceutical Co., Ltd.
Slc5a4b
MGI:1890478		solute carrier family 5 (neutral amino acid transporters, system A), member 4b10Slc5a4b deletion, Chr 10, Chugai Pharmaceutical Co., Ltd.
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse Slc5a4a genomic DNA, mouse Slc5a4b genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
    DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data