Strain Data Sheet

RBRC06162

Strain Information

Image
BRC No.RBRC06162
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ednra<tm1Ywa>
Former Common nameETA/129, B6-Ednra KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. JH1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background.
Strain descriptionETA (Ednra / endothelin receptor type A) gene knockout mice. Exons 5 and 6 were replaced with a neomycin resistant gene. Homozygous mutant mice are perinatal lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Cranial and cardiac neural crest defects in endothelin-A receptor-deficient mice.
Clouthier D E, Hosoda K, Richardson J A, Williams S C, Yanagisawa H, Kuwaki T, Kumada M, Hammer R E, Yanagisawa M
Development, 125, 813-824 (1998). 9449664

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ednra
MGI:105923		endothelin receptor type A8Ednra<tm1Ywa>
MGI:1857473		targeted mutation 1, Masashi Yanagisawa
  • mandibulofacial dysostosis with alopecia(DisGeNET, MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • GH Growth hormone polyA (Bovine)8GH
    neo neomycin resistance gene (E. coli)8 mouse RNA polymerase II promoter

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • aberrant origin of the right subclavian artery(MP:0010465)

  • abnormal Meckel's cartilage morphology(MP:0005587)

  • abnormal alisphenoid bone morphology(MP:0003235)

  • abnormal artery morphology(MP:0002191)

  • abnormal ascending aorta morphology(MP:0009867)
    • more 53 phenotypes
  • abnormal cardiovascular system morphology(MP:0002127)

  • abnormal carotid artery morphology(MP:0004181)

  • abnormal dorsal aorta morphology(MP:0004787)

  • abnormal facial nerve morphology(MP:0001071)

  • abnormal first pharyngeal arch morphology(MP:0006337)

  • abnormal fourth pharyngeal arch artery morphology(MP:0006354)

  • abnormal mandible morphology(MP:0000458)

  • abnormal mandibular nerve branching(MP:0001068)

  • abnormal maxilla morphology(MP:0000455)

  • abnormal middle ear ossicle morphology(MP:0005105)

  • abnormal mouth floor morphology(MP:0013262)

  • abnormal mouth morphology(MP:0000452)

  • abnormal muscle morphology(MP:0002108)

  • abnormal outer ear morphology(MP:0002177)

  • abnormal palate morphology(MP:0003755)

  • abnormal palatine bone morphology(MP:0005249)

  • abnormal pharyngeal arch artery morphology(MP:0002672)

  • abnormal pulmonary ventilation(MP:0002338)

  • abnormal sixth pharyngeal arch artery morphology(MP:0006355)

  • abnormal skeleton morphology(MP:0005508)

  • abnormal styloid process morphology(MP:0008023)

  • abnormal third pharyngeal arch artery morphology(MP:0006356)

  • abnormal trigeminal nerve morphology(MP:0001065)

  • absent Meckel's cartilage(MP:0004439)

  • absent gonial bone(MP:0008381)

  • absent incus(MP:0004318)

  • absent malleus(MP:0004319)

  • absent right subclavian artery(MP:0010486)

  • absent salivary gland(MP:0000614)

  • absent stapes(MP:0004204)

  • absent submandibular gland(MP:0009524)

  • absent tubotympanic recess(MP:0030228)

  • absent tympanic membrane(MP:0006019)

  • absent tympanic ring(MP:0003138)

  • aorta tubular hypoplasia(MP:0010543)

  • cleft chin(MP:0000114)

  • double aortic arch(MP:0004159)

  • double outlet right ventricle(MP:0000284)

  • ectopic thymus(MP:0003007)

  • interrupted aorta(MP:0010544)

  • lower jaw to upper jaw transformation(MP:0030299)

  • mandible hypoplasia(MP:0000460)

  • overriding aortic valve(MP:0000273)

  • patent ductus arteriosus(MP:0003139)

  • perimembraneous ventricular septal defect(MP:0010418)

  • perinatal lethality, complete penetrance(MP:0011089)

  • persistent right dorsal aorta(MP:0010572)

  • persistent truncus arteriosus(MP:0002633)

  • small second pharyngeal arch(MP:0006344)

  • small zygomatic bone(MP:0004468)

  • thymus hypoplasia(MP:0001823)

  • tongue hypoplasia(MP:0009904)

  • transposition of great arteries(MP:0004110)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse RNA polymerase II promoter, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, mouse Ednra genomic DNA
    Research applicationDevelopmental Biology Research
    Hematological Research
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 813-824 (1998).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data