Strain Data Sheet

RBRC06164

Strain Information

Image
BRC No.RBRC06164
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Edn2<tm1Ywa>
Former Common nameET2/129, B6-Edn2 KO
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background.
Strain descriptionET-2 (Edn2 / endothelin 2) gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a neomycin resistant gene. Homozygous mutant mice die after birth (3-4 weeks of age).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6J(Crlj)]
References
Endothelin-2 deficiency causes growth retardation, hypothermia, and emphysema in mice.
Chang I, Bramall A N, Baynash A G, Rattner A, Rakheja D, Post M, Joza S, McKerlie C, Stewart D J, McInnes R R, Yanagisawa M
J. Clin Invest., 123, 2643-2653 (2013). 23676500

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Edn2
MGI:95284		endothelin 24Edn2<tm1Ywa>
MGI:5519239		targeted mutation 1, Masashi Yanagisawa
GH Growth hormone polyA (Bovine)4GH
neo neomycin resistance gene (E. coli)4 mouse RNA polymerase II promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal blood homeostasis(MP:0009642)

  • abnormal brown adipose tissue morphology(MP:0002971)

  • abnormal gas homeostasis(MP:0003948)

  • abnormal lipolysis(MP:0008032)

  • abnormal pulmonary alveolar sac morphology(MP:0010902)
    • more 24 phenotypes
  • abnormal pulmonary alveolus morphology(MP:0002270)

  • acidemia(MP:0003030)

  • acidosis(MP:0003031)

  • decreased body size(MP:0001265)

  • decreased body temperature(MP:0005534)

  • decreased body weight(MP:0001262)

  • decreased circulating glucose level(MP:0005560)

  • decreased circulating triiodothyronine level(MP:0005479)

  • digestive/alimentary phenotype(MP:0005381)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hypercapnia(MP:0002318)

  • hypoxia(MP:0005039)

  • impaired adaptive thermogenesis(MP:0011049)

  • increased blood urea nitrogen level(MP:0005565)

  • increased blood uric acid level(MP:0008821)

  • increased circulating bicarbonate level(MP:0003016)

  • increased circulating erythropoietin level(MP:0011285)

  • increased circulating ketone body level(MP:0002575)

  • increased hematocrit(MP:0002608)

  • increased susceptibility to induced morbidity/mortality(MP:0009763)

  • increased total lung capacity(MP:0010937)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse RNA polymerase II promoter, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, mouse Edn2 genomic DNA
    Research applicationDevelopmental Biology Research
    Hematological Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin Invest., 123, 2643-2653 (2013). Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data