Strain Data Sheet

RBRC06171

Strain Information

Image
BRC No.RBRC06171
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Invs<inv> Tg(EF1-Invs/EGFP)B27Hmd
Former Common nameFVB,Inv::GFP-rescue, FVB-inv/invGFP#B27
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Daisuke Watanabe, School of Science, Kitasato University. The InvGFP transgene was injected into the pronuclei of FVB-Invs<inv> fertilized eggs. Tg mice of FVB background (RBRC06171) were crossed to ICR for 5 times (RBRC06172).
Strain descriptionInv rescued mice having a inv mutation and Inv::GFP fusion rescue gene. FVB background (RBRC06171), ICR background (RBRC06172). In the ICR background, unique phenotypes such as corneal thickening and spine curvature were observed.
Colony maintenanceInv: Heterozygote x Wild-type; Tg: Carrier x Carrier [or Crossing to FVB/NJcl]
References
Reversal of left-right asymmetry: a situs inversus mutation.
Yokoyama T, Copeland N G, Jenkins N A, Montgomery C A, Elder F F, Overbeek P A
Science. 260(5108):679-682 (1993). 8480178

The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia.
Watanabe D, Saijoh Y, Nonaka S, Sasaki G, Ikawa Y, Yokoyama T, Hamada H
Development, 130, 1725-1734 (2003). 12642479

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Invs
MGI:1335082		inversin4Invs<Invs>
MGI:1856915		inversion of embryonic turning
  • nephronophthisis 2(DisGeNET, MedGEN)
    • Tyr
    MGI:98880    		tyrosinase7Tyr
  • oculocutaneous albinism type 1A(MedGEN)

  • oculocutaneous albinism type 1B(MedGEN)

  • oculocutaneous albinism(DisGeNET)
    • EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
    Invs InversinUNInvs human elongation factor 1 alpha (EF-1 alpha) promoter

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse tyrosinase minigene, human EEF1A1 promoter, mouse Invs cDNA, Jellyfish GFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 130, 1725-1734 (2003). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in case of application for any patents or for-profit use based o the results from the use of the BIOLOGICAL RESOURCE.
    DepositorDaisuke Watanabe (The Kitasato Institute)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data