Strain Data Sheet

RBRC06186

Strain Information

Image
BRC No.RBRC06186
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Tg(H1-RNAi:EGFP/CAG-HcRed1)4Osb
Former Common nameHc-red(RNAi), FVB-red/RNAi Tg#4
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Daisuke Watanabe, School of Science, Kitasato University. The pGtoR plasmid was injected into the pronuclei of FVB-Invs<inv> Tg(EF1-Invs/EGFP)B27Hmd fertilized eggs. Invs<inv> Tg(EF1-Invs/EGFP)B27Hmd genes were removed. FVB/N background.
Strain descriptionpGtoR shRNA gene transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to FVB/NJcl]
References
Small interfering RNA and gene silencing in transgenic mice and rats.
Hasuwa H, Kaseda K, Einarsdottir T, Okabe M
FEBS Lett., 532: 227-230 (2002). 12459495

The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia.
Watanabe D, Saijoh Y, Nonaka S, Sasaki G, Ikawa Y, Yokoyama T, Hamada H
Development, 130, 1725-1734 (2003). 12642479

Multiple renal cyst development but not situs abnormalities in transgenic RNAi mice against Inv::GFP rescue gene.
Kamijho Y, Shiozaki Y, Sakurai E, Hanaoka K, Watanabe D
PLoS One, 9(2):e89652 (2014). 24586938

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP human RNA polymerase III promoter
HcRed1 Red Fluorescent Protein (Heteractis crispa)UNHcRed1 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal sperm number(MP:0002673)

  • abnormal spermatid morphology(MP:0006380)

  • enlarged spleen(MP:0000691)

  • kidney cortex cyst(MP:0000522)

  • kidney cyst(MP:0003675)
    • more 4 phenotypes
  • kidney medulla cyst(MP:0011307)

  • postnatal lethality(MP:0002082)

  • renal glomerulus fibrosis(MP:0011377)

  • situs inversus(MP:0002766)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse EF1 promoter, mouse Invs cDNA, Jellyfish GFP cDNA, human H1 promoter, CMV IE enhancer, chicken beta-action promoter, rabbit beta-globin genomic DNA, Heteractis crispa HcRed cDNA
    Research applicationFluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 130, 1725-1734 (2003). PLoS One, 9(2):e89652 (2014). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in case of application for any patents or for-profit use based o the results from the use of the BIOLOGICAL RESOURCE.
    DepositorDaisuke Watanabe (The Kitasato Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data