Strain Data Sheet

RBRC06199

Strain Information

Image
BRC No.RBRC06199
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NDE-hsp-GCaMP2,lacZ)1Hmd
Former Common nameNDE-GCaMP2-IZ(1), B6-NDE-GCaMP2/lacZ Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Satoko Yoshiba and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The NDE-hsp- GCaMP2,lacZ transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6, 129 and C3H mixed background.
Strain descriptionThis transgenic mouse expresses GCaMP2 and lacZ genes on both sides of the node under the control of the mouse node-specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein.
Nakai J, Ohkura M, Imoto K
Nat. Biotechnol., 19, 137-141 (2001). 11175727

Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.
Yoshiba S, Shiratori H, Kuo I Y, Kawasumi A, Shinohara K, Nonaka S, Asai Y, Sasaki G, Belo J A, Sasaki H, Nakai J, Dworniczak B, Ehrlich B E, Pennekamp P, Hamada H
Science, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GCaMP2 RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2 mouse node-notochord enhancer, mouse Hsp68 promoter
Ires internal ribosomal entry site (EMCV)UN
lacZ beta-galactosidase (E. coli)UN
SV40 polyA signalUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse node-notochord enhancer, mouse Hsp68 promoter, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyA signal
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data