Strain Data Sheet

RBRC06203

Strain Information

Image
BRC No.RBRC06203
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pitx2<tm1.1Mbf>
Former Common namePitx2 A-17 flox, B6-Pitx2 floxed
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hidetaka Shiratori and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2001. G4 ES cells were used. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionPitx2 gene floxed mice. A loxP site was inserted in the intron 5, and a neo cassette with a 3’ loxP site was inserted downstream of intron 5. Homozygous mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Characterization of Pitx2c expression in the mouse heart using a reporter transgene.
Furtado M B, Biben C, Shiratori H, Hamada H, Harvey R P
Dev. Dyn., 240, 195-203 (2011). 21089073

Health Report

Examination Date / Room / Rack2024/12/09Room:3-5Rack:CSentinel mouse program
2024/09/09Room:3-5Rack:CSentinel mouse program
2024/06/10Room:3-5Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pitx2
MGI:109340		paired-like homeodomain transcription factor 23Pitx2<tm1.1Mbf>
MGI:4880738		targeted mutation 1.1, Milena B Furtado
  • anterior segment dysgenesis 4(MedGEN)

  • Axenfeld-Rieger syndrome type 1(DisGeNET, MedGEN)

  • ring dermoid of cornea(MedGEN)
    • loxP phage P1 loxP3loxP
    loxP phage P1 loxP3loxP
    neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse PGK1 promoter, E.coli neo gene, mouse PGK1 polyA signal sequence, mouse Pitx2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 240, 195-203 (2011).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityLive mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data