Strain Data Sheet

RBRC06210

Strain Information

Image
BRC No.RBRC06210
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pgap3<tm1>
Former Common namePgap3 floxed, B6-Pgap3 floxed
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Yetao Wang and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 2007. EGRG01 ES cells derived from (129S2 x C57BL/6CrSlc)F1-Tg(GFP) were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionPgap3 gene floxed mice. Exon 7 and 8 were flanked by loxP sites. A FRT flanked neo cassette was inserted after the exon 8.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins.
Murakami H, Wang Y, Hasuwa H, Maeda Y, Kinoshita T, Murakami Y
Biochem. Biophys. Res. Commun., 417, 1235-1241(2012). 22227195

Significance of glycosylphosphatidylinositol-anchored protein enrichment in lipid rafts for the control of autoimmunity.
Wang Y, Murakami Y, Yasui T, Wakana S, Kikutani H, Kinoshita T, Maeda Y
J. Biol. Chem., 88, 25490-25499 (2013). 23864655

Health Report

Examination Date / Room / Rack2024/10/07Room:3-BRack:ESentinel mouse program
2024/07/08Room:3-BRack:EAdditional tests
2024/04/08Room:3-BRack:ESentinel mouse program
2024/01/09Room:3-BRack:ESentinel mouse program
2023/10/10Room:3-BRack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
Pgap3
MGI:2444461		post-GPI attachment to proteins 311Pgap3<tm1Ymra>
MGI:5538656		targeted mutation 1, Yoshiko Murakami
  • hyperphosphatasia with intellectual disability syndrome 4(MedGEN)
    • loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast flippase target sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Pgap3 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorTaroh Kinoshita (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data