Strain Data Sheet

RBRC06212

Strain Information

Image
BRC No.RBRC06212
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bdnf<tm1.2Hko>
Former Common nameBDNF Delta FRT/+ (GWAS005), B6-Bdnf floxed
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. B6JN/1 ES cells derived from C57BL/6 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionBDNF (Bdnf) gene floxed mice. Exon 5 was flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bdnf
MGI:88145		brain derived neurotrophic factor2Bdnf<tm1.2Hko> targeted mutation 1.2, Haruhiko Koseki
Frt yeast FRT (flippase recombination target) site2Frt
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obese(MP:0001261)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bdnf genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data