Strain Data Sheet

RBRC06217

Strain Information

Image
BRC No.RBRC06217
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Slc39a13<tm1Thir>
Former Common nameZIP13 KO, B6-Slc39a13 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Toshiyuki Fukuda, RIKEN Center for Allergy and Immunology in 2008. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used for the generation of knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionZip13 (Slc39a13) gene knockout mice. Exons 6 through 8 were replaced with a neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
The zinc transporter SLC39A13/ZIP13 is required for connective tissue development; its involvement in BMP/TGF-beta signaling pathways.
Fukada T, Civic N, Furuichi T, Shimoda S, Mishima K, Higashiyama H, Idaira Y, Asada Y, Kitamura H, Yamasaki S, Hojyo S, Nakayama M, Ohara O, Koseki H, Dos Santos H G, Bonafe L, Ha-Vinh R, Zankl A, Unger S, Kraenzlin M E, Beckmann J S, Saito I, Rivolta C, Ikegawa S, Superti-Furga A, Hirano T
PLoS One. 3(11):e3642 (2008). 18985159

Zinc transporter ZIP13 suppresses beige adipocyte biogenesis and energy expenditure by regulating C/EBP-beta expression.
Fukunaka A, Fukada T, Bhin J, Suzuki L, Tsuzuki T, Takamine Y, Bin B H, Yoshihara T, Ichinoseki-Sekine N, Naito H, Miyatsuka T, Takamiya S, Sasaki T, Inagaki T, Kitamura T, Kajimura S, Watada H, Fujitani Y
PLoS Genet., 13(8):e1006950 (2017). 28854265

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slc39a13
MGI:1915677		solute carrier family 39 (metal ion transporter), member 132Slc39a13<tm1Thir>
MGI:3829756		targeted mutation 1, Toshio Hirano
  • Ehlers-Danlos syndrome, spondylocheirodysplastic type(MedGEN)
    • neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal alveolar process morphology(MP:0004189)

  • abnormal bone structure(MP:0003795)

  • abnormal dermal layer morphology(MP:0001243)

  • abnormal epiphyseal plate morphology(MP:0006395)

  • abnormal eyelid aperture(MP:0003671)
    • more 23 phenotypes
  • abnormal incisor morphology(MP:0005358)

  • abnormal jaw morphology(MP:0000454)

  • abnormal long bone epiphyseal plate proliferative zone(MP:0003662)

  • abnormal long bone epiphysis morphology(MP:0000131)

  • abnormal long bone hypertrophic chondrocyte zone(MP:0000165)

  • abnormal long bone metaphysis morphology(MP:0000133)

  • abnormal long bone morphology(MP:0003723)

  • abnormal mandible morphology(MP:0000458)

  • abnormal molar morphology(MP:0002689)

  • abnormal osteoblast physiology(MP:0005006)

  • abnormal tooth morphology(MP:0002100)

  • abnormal trabecular bone morphology(MP:0000130)

  • decreased bone mineral density(MP:0000063)

  • decreased compact bone thickness(MP:0000135)

  • decreased cornea thickness(MP:0005543)

  • decreased length of long bones(MP:0004686)

  • enophthalmos(MP:0006198)

  • kyphosis(MP:0000160)

  • malocclusion(MP:0000120)

  • postnatal growth retardation(MP:0001732)

  • small mandible(MP:0004592)

  • small maxilla(MP:0004540)

  • thickened long bone epiphysis(MP:0000132)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Slc39a13 genomic DNA
    Research applicationDermatology Research
    Developmental Biology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 3(11):e3642 (2008). Prior to requesting the BIOLOGICAL RESOURCE, the Recipient shall contact with the Developer scientist (Dr. Toshiyuki Fukada, Tokushima Bunri University, email: fukada@ph.bunri-u.ac.jp) and obtain the use permission.
    DepositorDrs. Shigeo Koyasu and Toshiyuki Fukada (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hirose T, Shimazaki T, Takahashi N, Fukada T, Watanabe T, Tangkawattana P, Takehana K.
    Morphometric analysis of thoracic aorta in Slc39a13/Zip13-KO mice.
    Cell Tissue Res 376(1) 137-141(2019) 30610452
    Hirose T, Suzuki I, Takahashi N, Fukada T, Tangkawattana P, Takehana K.
    Morphometric analysis of cornea in the Slc39a13/Zip13-knockout mice.
    J Vet Med Sci 80(5) 814-818(2018) 29563392