Strain Data Sheet

RBRC06218

Strain Information

Image
BRC No.RBRC06218
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Susd3<tm1(KOMP)Vlcg>
Former Common nameSusd3 KO, B6-Susd3 KO
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain developmentDeveloped by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University. The original allele and mouse were on C57BL/6 background and from the Knockout Mouse Project (KOMP) Repository.
Strain descriptionSusd3, sushi domain containing 3 gene knockout mice. A 17004 bp region between 49,343,498-49,326,495 positions of Chromosome 13 was deleted by the insertion of ZEN-Ub1 cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pgk1 mouse PGK polyA signal13Pgk1
Susd3
MGI:1913579		sushi domain containing 313Susd3<tm1(KOMP)Vlcg>
MGI:5292886		targeted mutation 1, Velocigene
lacZ beta-galactosidase (E. coli)13
loxP phage P1 loxP13loxP
loxP phage P1 loxP13loxP
neo neomycin resistance gene (E. coli)13 human ubiquitin C promoter
SV40 polyA signal13

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAVelocigene cassette ZEN-Ub1(E. coli Beta-galactosidase, SV40 polyA, phage P1 loxP sites, human ubiquitin C promoter, E. coli neoR gene, mouse PGK polyA), mouse Susd3 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorTakahisa Furukawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data