Strain Data Sheet

RBRC06237

Strain Information

Image
BRC No.RBRC06237
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Capn6<tm1Hiso>
Former Common nameB6-Capn6 KO
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuo Tonami and Hiroki Kurihara, Graduate School of Medicine, The Tokyo University in 2004. Maintained by Tokyo Metropolitan Institute of Medical Science. G4 ES cells were used. The mutant mice were crossed to C57BL/6 (RBRC06237) and ICR (RBRC06238).
Strain descriptionCapn6 gene knockout mice. Exon 2 of Capn6 was replaced with a nls-lacZ/PGK-neo cassette. Capn6 deficient mice (homozygous females and hemizygous male) were viable, but display precocious development of embryonic skeletal muscle and promote the differentiation of skeletal muscle progenitors cells. X linked. C57BL/6 background (RBRC06237) and ICR mixed background (RBRC06238).
Colony maintenanceHomozygote (female) x Hemizygote (male) [or Crossing to C57BL/6J(Crj)]
References
Calpain-6 deficiency promotes skeletal muscle development and regeneration.
Tonami K, Hata S, Ojima K, Ono Y, Kurihara Y, Amano T, Sato T, Kawamura Y, Kurihara H, Sorimachi H
PLoS Genet., 9(8):e1003668 (p1-11) (2013). 23935533

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Capn6
MGI:1100850		calpain 6XCapn6<tm1Hiso>
MGI:5544425		targeted mutation 1, Hiroyuki Sorimachi
lacZ beta-galactosidase (E. coli)X
lox71 mutant lox sequenceXlox71
neo neomycin resistance gene (E. coli)X mouse phosphoglycerate kinase promoter (PGK promoter)
nls SV40 large T antigen nuclear localization signal (NLS)Xnls
SV40 polyA signalX

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal muscle regeneration(MP:0000750)

  • abnormal myogenesis(MP:0000729)

  • abnormal skeletal muscle regeneration(MP:0013237)

  • abnormal tongue muscle morphology(MP:0004136)

  • increased skeletal muscle fiber size(MP:0009399)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 mutant loxP sequence, SV40 nls (nuclear localization signal), E. coli lacZ gene, SV40 PolyA signal, Mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, bovine growth hormone 1 poly A, Mouse Capn6 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 9(8):e1003668 (p1-11) (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
    In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues (Kazuo Tonami, Hiroki Kurihara, and Hiroyuki Sorimachi) are included as co-authors.
    DepositorHiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data